Biocatalyst Immobilization and Modification

Biocatalyst Immobilization and Modification
Besides protein engineering, chemical approaches including chemical modification and immobilization are employed in designing biocatalysts with desirable bio-properties for industrial applications. Enzyme immobilization, first commercialized in the 1960s, has been well developed to facilitate the reusability and recovery of enzymes. High stabilization of a biocatalyst, such as pH and thermal stability, can often be achieved through immobilization, and enhanced activities are demonstrated in an increasing number of recent reports.
Immobilization can be generally categorized into carrier-bound immobilization and carrier-free immobilization. In carrier-bound applications, solid support attachment is a typical example, where enzymes are attached in solid surfaces either through physical adsorption or covalent linkages. Entrapment and cross-linking are other alternative methods in this category. In carrier-free applications, cross-linked enzyme aggregates (CLEAs) are developed by simple precipitation of the enzyme from a solution by adding salt, (such as ammonium sulfate), or a water-miscible organic solvent, followed by cross-linking with a bifunctional reagent. This method has been demonstrated to be sufficient for two or more enzymes and known as combi-CLEA.
Creative Enzymes provide the best biocatalyst immobilization and modification services for researchers. With years of experience in this field, we offer professional one-stop services with reliable outcomes, improved performance, and biophysical analysis:
Custom biocatalyst immobilization design;
Process optimization;
Activity and stability measurements;
Process scaling-up.
https://www.creative-enzymes.com/service/biocatalyst-immobilization-and-modification_409.html
Synzymes
Creative Enzymes brings cutting-edge technologies to solve the unique demands in your enzyme research. Creative Enzymes performs contract research, through professional and specialized knowledge. In addition to native and recombinant enzymes, we also design and produce artificial enzymes. Our synzymes service include the design and synthetic process as well as the subsequently enzyme activity analysis.
Specialized technologies in design, synthesis, and production
Rapid turnaround
Template based or de novo design
Highly customized for each request

Biocatalyst Immobilization and Modification